Fig. 5

Fig. 5 Sperm matured at high temperature showed an abnormality.

a Flow cytometric analysis of sperm DNA content. PI luminance values (30–42) were set as the P4 gate. b Percentage of sperm within the P4 gate. Values are the mean ± SEM (n = 6). *p < 0.01 compared with the 29 °C sperm. c Fertilization rates of 34 °C exposed male. Values are the mean ± SEM (29 °C n = 4 and 34 °C n = 5). **p < 0.01 and *p < 0.05 compared two groups. d Survival rates of embryos derived from 34 °C exposed males. Values are the mean ± SEM (29 °C n = 4 and 34 °C n = 5). *p < 0.01 compared with the 29 °C sperm. e Percentage of morphologically abnormal embryos derived from 34 °C exposed males. Values are the mean ± SEM (29 °C n = 4 and 34 °C n = 5). *p < 0.01 compared with the 29 °C sperm. f Images of embryos at 2 dpf and 6 dpf. Scale bars are 500 μm.