Fig 6

Fig 6

TRAF3 overexpression reduces survival and promotes fungal load in vivo in A. fumigatus-infected zebrafish. (A) Images of zebrafish larvae transgenic for TRAF3 and expressing EGFP at 3 dpf. qRT-PCR was used to detect the transcriptional expression levels of TRAF3 at various time points after zebrafish infection with A. fumigatus (B). Zebrafish larvae were immersed in E2 medium containing 1 × 10⁷A. fumigatus spores and replaced with sterile E2 medium after 2 h. The effect of TRAF3 on the survival rate of zebrafish larvae infected with A. fumigatus (C) and the fungal load of zebrafish larvae at various time points (D) were examined.The expression levels of pro-inflammatory cytokines (E–H) in zebrafish infected with A. fumigatus at various time points were measured by qRT-PCR. ns stands for no statistically significant difference; *, # P < 0.05; **P < 0.01; ***P < 0.001. Scale bars: 50 µm.