ZFIN Image: Lee et al., 2023, Figure 2

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Figure 2

Promoter activity driven by the −2055~+77 DNA fragment of human ENDOU-1 could be induced by various stress-inducing drugs. (A) HEK-293T cells co-transfected with pE2.1p and phRG-TK plasmid for 24 h and then treated with DMSO, Thapsigargin (TH), Anisomycin (Am), CoCl₂ (Co), Poly I:C (IC), or CuSO₄ (Cu) for 6 h, followed by analysis of luc activity via a dual-luc assay. Cells treated with DMSO served as a control group. The relative luc activity was represented by the fold increase in the Flu/Rlu ratio over that obtained from the pCS2-transfected control group, normalized as 1. The values were averaged from three independent experiments and presented as mean ± S.D. (n = 3). The Student’s t-test was used to perform statistical analysis (***, p < 0.001; **, p < 0.005) (B) Western blot analysis of proteins, as indicated, in HEK-293T cells. The α-tubulin served as internal control.

Acknowledgments

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