Figure 5

Figure 5

Clasp2 transcript distribution in sections of adult guppy testis. (a) In this section, we identified the organization of the testis. Cysts containing germ cells in the early stages of spermatogenesis (spermatogonia and spermatocytes) are located near the periphery of the testis, while cysts with numerous spermatids at different stages of spermiogenesis are located deeper, near the efferent ducts. In the same region, there are present spermatozeugmata cysts, in which spermatozoa are tightly packed with the sperm heads oriented towards the Sertoli cells and flagella oriented towards the center of the cyst. Spermatozeugmata is also found inside the efferent ducts (located in the central region of the testis), where mature spermatozoa are released. Chromogenic in situ hybridization of clasp2 in this section shows a clear marking at the periphery of the spermatozeugmata. The peripheral region of spermatozeugmata cysts located inside the efferent ducts shows weak staining (Sg: spermatogonia; Sc: spermatocytes; St: spermatids; Sz: spermatozeugmata; Ed: efferent ducts). (b) The strong marking of spermatozeugmata. (Sz: spermatozeugmata). Scale bars: 100 µ (a); 50 µ (b).