|
Fig. 5 Tissue distribution of the six collagen IV α chains in zebrafish development. (A) In situ hybridization for zebrafish α6 chains at 36 hpf in the brain (b), otic vesicle (o), gills (g), developing cardiac tissue (c), and the pronephric duct (pn) of the future kidney. (B) RT-PCR products in adult zebrafish for the six α chains are shown above the positive control β-actin. All six α chains are detectable in brain, gills, kidney, skeletal muscle, swim bladder, and testes. Gut/bowel and skin express the α1, α2, α5, and α6 chains only. Liver and heart both display expression of all α chains except the α3 chain.