ZFIN Image: Hagedorn et al., 2023, Fig. 3

Image

Figure Caption

Fig. 3 CHT- and pan-endothelial-specific regulatory elements

(A) Image and schematic depict the four cell populations that were isolated from mrc1a 1.3kb:GFP⁺; kdrl:mCherry⁺ double-positive embryos for analysis by ATAC-seq.

(B) Gene tracks show regions of chromatin that were uniquely open in the GFP⁺mCherry⁺ CHT EC fraction. Images on the right show embryos injected with a CHT EC enhancer-GFP reporter construct corresponding to the red boxed region (red arrow). Arrowheads points to GFP expression in CHT ECs.

(C) Gene tracks show regions of chromatin that were open in both the mCherry⁺GFP⁺ (CHT EC) and mCherry⁺GFP⁻ (non-CHT EC) populations (red boxes and arrows). Images on the right show embryos injected with pan-endothelial enhancer-GFP reporter constructs corresponding to the red boxed regions. Arrows point to GFP expression in non-CHT ECs and arrowheads point to expression in CHT ECs.

(D) Images show reporter expression of stable enhancer-GFP transgenes. Arrows point to GFP expression in non-CHT ECs and arrowheads point to expression in CHT ECs.

Acknowledgments

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Reprinted from Developmental Cell, 58(12), Hagedorn, E.J., Perlin, J.R., Freeman, R.J., Wattrus, S.J., Han, T., Mao, C., Kim, J.W., Fernández-Maestre, I., Daily, M.L., D'Amato, C., Fairchild, M.J., Riquelme, R., Li, B., Ragoonanan, D.A.V.E., Enkhbayar, K., Henault, E.L., Wang, H.G., Redfield, S.E., Collins, S.H., Lichtig, A., Yang, S., Zhou, Y., Kunar, B., Gomez-Salinero, J.M., Dinh, T.T., Pan, J., Holler, K., Feldman, H.A., Butcher, E.C., van Oudenaarden, A., Rafii, S., Junker, J.P., Zon, L.I., Transcription factor induction of vascular blood stem cell niches in vivo, 1037-1051.e4, Copyright (2023) with permission from Elsevier. Full text @ Dev. Cell