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Fig. S1 Light activation of Cilia-bPAC and Cyto-bPAC does not morphologically perturb development, related to Figure 1
(A) Immunoblot for Myc-tagged Cyto-bPAC and Cilia-bPAC in 24hpf zebrafish embryo lysates. β-actin serves as a loading control.
(B) Whole-mount brightfield images of transgenic Cyto-bPAC and Cilia-bPAC zebrafish embryos either raised in the dark or stimulated with blue light from 14-18 hours post fertilization.
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Reprinted from Cell, 184(11), Truong, M.E., Bilekova, S., Choksi, S.P., Li, W., Bugaj, L.J., Xu, K., Reiter, J.F., Vertebrate cells differentially interpret ciliary and extraciliary cAMP, 2911-2926.e18, Copyright (2021) with permission from Elsevier. Full text @ Cell