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Figure 5

ID: ZDB-IMAGE-231016-22
Publication: Shrestha et al., 2023 - The Effects of Aging on Rod Bipolar Cell Ribbon Synapses
All Figures: Figures for Shrestha et al., 2023

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Figure 5

The local Ca²⁺ responses elicited by brief depolarization at an Mb1 bipolar cell synaptic ribbon were altered in older-aged zebrafish. (A,B) Average Cal520-2LA fluorescence at ribbon locations in response to 10 ms depolarization in Mb1 bipolar cells isolated from middle-aged (MA; left panels; n = 3 ribbons from 3 cells and fish) and older-aged (OA; right panels; n = 3 ribbons from 3 cells and fish) zebrafish with pipette solution containing 2 mM EGTA. Shown is the change in fluorescence (ΔF) from baseline before stimulation, divided by the baseline fluorescence (F_min). (C,D) Same as in (A,B), except that the pipette solution contained 10 mM EGTA. (E,F) Same as in A-B, except that the pipette solution contained 2 mM BAPTA. Red arrows indicate the time of pulse stimulation.

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