ZFIN Image: Man et al., 2023, Figure 6

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Figure 6

The majority of opn5⁺ and opn8⁺ cells were activated in response to light. (A,C) Double FISH against opn5 (red) and c-fos (green) (A) and opn8 (red) and c-fos (green) (C) on stage 43/44 Xenopus laevis transverse retinal sections after 30 min of light stimulation. DAPI (blue) stains the nucleus. White arrows point to opn5⁺ or opn8⁺ cells that do not express c-fos, while yellow arrows point to opn5⁺ or opn8⁺ cells that are also c-fos⁺. GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer. Scale bar = 50 μm. (B,D) Quantification and distribution between the INL and GCL of cells co-expressing opn5 and c-fos (B) and opn8 and c-fos(D). Each dot represents the percentage of cells in one central retina section n = 12 sections; Lines are the mean with 95% confidence interval.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Cell. Neurosci.