ZFIN Image: Zizioli et al., 2023, Figure 3

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Figure Caption

Figure 3

Rescue of the phenotype in neu3.2 morphants with Mus musculus Neu2 mRNA. Representative images, at 72 hpf, of the injected embryos with STD-MO (A) and with neu3.2 MO (1 pmol/embryo) alone (B) or together with 50 pg/embryo Neu2 mouse mRNA (C). The percentage of embryos with the different phenotypes (normal and severe) in the three categories of the analyzed embryos is shown in the graph (C). Neu 3.2 activity measured in the soluble fraction using 4-MU-NeuAc as a substrate at a pH = 5.6 in all three categories of analyzed embryos is shown in the graph (D). Three experiments were performed and more than 150 embryos of each type were analyzed. (**** p < 0.0001 vs. injected embryos with standard morpholino ST-MO—Unpaired; two-tailed t-test).

Acknowledgments

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