Fig. 4

No corneal abnormity was observed in miR-184^−/− zebrafish. (A) The anterior corneal morphology of 3-month-old miR-184^−/− zebrafish was observed with a AS-OCT. L, lens; C, cornea; Ir, iris. (B) The cornea development was detected in embryonic WT and miR-184^−/− zebrafish. The corneal epithelia were labeled with DAPI staining, while the stroma was stained with Alexa Fluor™ 594-conjugated WGA. L, lens; C, cornea. Bar, 25 μm. (C and D) The corneal structure (C) and thickness (D) were detected in 5-month-old WT and miR-184^−/− zebrafish. Student t-test was used for statistical analysis, n = 3. ns, no significant. Bar, 50 μm. (E and F) The corneal structure (E) and thickness (F) were detected in 1-year-old WT and miR-184^−/− zebrafish. Student t-test was used for statistical analysis, n = 4. ns, no significant. Bar, 50 μm.