ZFIN Image: Farre et al., 2023, FIGURE 3

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Figure Caption

FIGURE 3

Expression of gngt2a in adult wildtype zebrafish retina using multiplex fluorescence in situ hybridization chain reaction (HCR). (A) Expression of lws1 (magenta) and lws2 (cyan) in a representative whole retina (D, dorsal). (A’) Expression of gngt2a (yellow) in the same preparation, showing that signal intensity appears greatest in the lws1-expressing domain, but signal is not confined to this domain. (A”)gngt2a and lws1. (A”’)gngt2a and lws2. (B) 40x image of lws1 and lws2 expression in a region of transition from lws1 to lws2. (B’) 40x image of gngt2a expression in the same region, indicating gngt2a is not restricted to lws1-expressing cones, nor to LWS cones in general. (C) Selected enlarged region of (A”) showing gngt2a and lws1. (D) Same region showing gngt2a and lws2. (D–D”’) Resliced orthogonal projections of (B). (D) All imaging channels merged. (D’) DAPI and gngt2a.(D”) DAPI and lws1. (D”’) DAPI and lws2. gngt2a is co-expressed by lws1 + and lws2 + cones, as well as in non-LWS cones. Sample size = 2.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Cell. Neurosci.