Figure 8—figure supplement 2.

Figure 8—figure supplement 2. Ca²⁺ response of neighboring cells to Opto-ASC induced basal extrusion and its quantification.

(A) Example of Ca²⁺ signaling in epithelial cells for basally extruding periderm cells imaged at high temporal resolution (15 frames per minute). Opto-ASC (magenta) is induced in a periderm cell in the center of the frame by blue light (488 nm). Both the speck and the Ca²⁺ signal in the neighboring cell appear simultaneously at 00:49 min. The stages of cell death are indicated as defined in Figure 5: shrinking in yellow, internalizing in green, and fragmentation in blue. (B–C) Ca²⁺ responses in cells adjacent to the dying cell. Number of neighboring cells that show a Ca²⁺ elevation in response to Opto-ASC-induced basal extrusion and Opto-Caspase-8-induced apoptosis. Three movies, indicated by triangles, squares and circles, were analyzed in each experiment. Number of 1^st neighbors (cells directly in contact with the dying cell) to Opto-ASC-induced dying cells (B) and to Opto-Caspase-8-induced dying cells (C) plotted over time. periderm cells are represented in black, and basal cells are in red.