Image
Figure Caption

Figure 7—figure supplement 2. Transient expression of HSE: GFP/ASC-mKate2 in basal cells.

(A) Bright field image of the skin of a caspa-/- larvae showing the outlines of periderm cells (yellow) which were identified by scanning though the z-planes of the stack. Scale bar is 20 µm. (B) Time laps of basal cells transiently expressing HSE: GFP/ASC-mKate2 underlying the periderm cells identified in A and outlines in yellow. GFP is in green, ASC.mKate2 in magenta. Scale bar is 20 µm. (C) Time between speck formation and cell death measure in minutes in basal cells of larvae of the tg(HSE:Opto-Asc) (N=23) lines compared to basal cells in transiently expressing caspa-/- (N=20) larvae.

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