ZFIN Image: Hasel de Carvalho et al., 2023, Figure 5.

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Figure Caption

Figure 5. Stages of ASC-speck-induced e extrusion from the periderm.

(A) Example of a single cell from a bright-field movie (top) and a schematic showing stages of periderm cell extrusion and lysis after speck formation (bottom). (B) DRAQ7 staining of a cell that lyses after the full extrusion from the periderm. Scale bar is 20 µm. (C) Time lines for 10 periderm cells undergoing the steps of cell extrusion described in A. Time is in minutes. (D) Basal cell dying after formation of an Opto-ASC-speck (magenta). Cell membrane is labeled by AKT-PH-GFP (green). (E) Time between Opto-ASC speck formation and the appearance of first morphological signs of cell death (deformation of cell) in periderm (N=49) and basal cells (N=20). ****=p < 0.0001.

Acknowledgments

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