Figure 7—figure supplement 1.

Figure 7—figure supplement 1. Inflammatory caspases in cell extrusion and rapid cell death after ASC-speck formation. (A) Schematic of the caspa and caspb genes and sgRNA binding sites for F0 CRISPR/Cas9 knock-outs (F0 KO). (B) Representative responses of periderm cells after speck formation in caspa F0 KO, caspb F0 KO and caspa F0 KO/caspb F0 KO. Scale bar is 20 µm. (C) Percentages of apically extruded and basally extruded cells and cells that do not die within the observation time of >60 min after Opto-ASC speck formation in different F0 KO backgrounds. (D) Periderm cell dying by apoptosis after Opto-Caspase-8 induction; scale bar is 20 µm. (E) Schematic of basally extruding cell after Opto-ASC induction and isomorphic shrinkage as seen in in caspb F0 KO larvae after Opto-ASC induction. (F) Time between Opto-ASC speck formation and first morphological signs of cell death (deformation of cell) in the indicated conditions. (G) Time of gap closure after death of single periderm cells which are either extruded or retained after the indicated treatments. (H) Number of fragments at the time of gap closure and area of the largest cell fragment of retained cells after ASC-Speck formation in WT and caspb F0 KO larvae and of apoptotic cells after Opto-Caspase-8 induction. (F–H) **=p < 0.01, ***=p < 0.001, ****=p < 0.0001.