Figure 6—figure supplement 3.

Figure 6—figure supplement 3. Actin dynamics in neighboring cells after Opto-ASC-induced cell death in basal cells.

(A, B) Tg(Opto-asc) (magenta) was crossed with Tg(UAS:mNG-UtrCH) to label actin (shown in cyan). (A) Opto-ASC is induced in a basal cell. The speck is seen within 1 min. Actin concentrates at the periphery of the dying cell, but it is not clear whether it is in the dying or the neighboring cells. No actin rearrangement is observed in the periderm above the dying basal cell. (B) An Opto-ASC speck is induced in the central basal cell which does not express UAS:mNG-UtrCH. The speck is first seen at 14:30 min. The surrounding cells form lamellipodia that fill in the gap above the dying cell, but no strong actin concentration is seen, indicating that the actin at the periphery of the cell in panel A is the actin of the dying cell. Scale bar: 20 μm.