Figure 6—figure supplement 1.

Figure 6—figure supplement 1. Response of neighbouring cells to cell death after ASC-speck formation.

(A) Response of macrophage to a basally extruded periderm cell (Opto-ASC in magenta). The macrophage (marked in yellow dashed outline) moves towards the dying cell which starts shrinking at 1:19 min, and engulfs the cell completely by 22:22 min. The macrophage is labelled with mpeg-gfp, and the periderm cell membranes with AKT-PH-GFP (both green). The scale bar is 20 µm. (B) Response of the surrounding periderm to a basally extruding periderm cell after Opto-ASC (magenta) speck formation. The white box marks the region of contact between the dying cell and a neighboring periderm cell. The same area is shown at higher magnification below with only the membrane label in grey scale. Blebs start to form as the dying cell disintegrates, and the remaining cell debris appears to be taken up by the surrounding cells. Actin is labelled by mNeonGreen-UtrCH (cyan) and the cell membranes by lyn-tagRFP (magenta).