ZFIN Image: Hasel de Carvalho et al., 2023, Figure 4.

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Figure Caption

Figure 4. Opto-ASC speck formation and dependence on endogenous ASC.

(A, B) Speck formation induced by ASC-mKate2, Opto-ASC, Opto-PYD and Opto-CARD in wildtype larvae and asc^Δ2/Δ2 larvae. Scale bar is 20 µm. (A) Time for ASC-induced specks is counted from end of heat shock. Purple arrow heads mark the speck in the cell of interest. Specks formed by surrounding cells can be seen as black spots. (B) Time is in min counted from the last timepoint before visible speck formation. (C) Time is measured between first detectable appearance of a speck and first morphological sign of cell death (deformation of cell) in wildtype (N=23) and asc^Δ2/Δ2 (N=14) larvae. *=p < 0.05. (D) Dynamics of speck formation in three cells with different expression levels of Opto-PYD. Relative intensity levels shown in the diagram on the left are measured in relation to the cell with the lowest level (1x) after background subtraction. Time is measured in minutes; scale bar is 20 µm.

Acknowledgments

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