Figure 3—figure supplement 1.

Figure 3—figure supplement 1. Recruitment dynamics in Opto-ASC specks and ASC-mKate2 specks.

(A) Recruitment dynamics of endogenous ASC (ASC-GFP) and Opto-ASC to a speck induced by blue light (488 nm). MFI was measured for GFP and mCherry at each time point over the entire area of the growing specks. Representative curve for 10 specks measured in three different larvae. (B) Recruitment of ASC-mKate2 and Opto-ASC in ASC-mKate2 specks and Opto-ASC specks. MFI of mKate2 and mCherry was measured at each point over the entire area of the growing speck and the time point at which the MFI value was constant was recorded. (C) Overlap of Opto-ASC (magenta) and endogenous ASC-GFP in light-induced speck at high resolution (Airyscan image acquired by Zeiss LSM 880). (D) Overlap of ASC-mKate2 (magenta) speck with endogenous ASC (GFP). The line ROI is drawn across the two specks and the normalized grey values are plotted along the line ROI. The distribution of the endogenously tagged Opto-ASC with ASC-GFP is uniform similar to that of the speck of ASC-mKate2 and ASC-GFP. (E) Comparison of diameter (in μm) of Opto-ASC specks (=11) and ASC-mKate2 specks (n=8).