Figure 6

Figure 6

Toxic effects of BPF on oligodendrocytes of zebrafish larvae at 5 dpf. (A–C) Lateral views of the spinal cord of Tg(MBP:EGFP) at 5 dpf. The oligodendrocytes expressed EGFP fluorescence. (D) The graph compares the number of oligodendrocytes per three somites in 0.1% DMSO and 5, 10 mg/L BPF-treated zebrafish. n = 10 for DMSO control and BPF 10 mg/L, n = 13 for BPF 5 mg/L. (E,F) The myelin sheaths were expressed in GFP in Tg(ClaudinK:mGFP). (G) The graph compares the average length of myelin sheaths per oligodendrocyte in 0.1% DMSO and zebrafish treated with 10 mg/L BPF. n = 15 for DMSO control, n = 10 for BPF 10 mg/L. Data are presented as means ± SD. n.s.—not significant, ** p < 0.01. (A–C) Scale bar, 100 µm. (E–F) Scale bar, 50 µm.