ZFIN Image: Sun et al., 2023, Fig. 5

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Figure Caption

Fig. 5 Coronary re-growth follows and requires hapln1a+ cell structures.

a Whole-mount view of coronary vessels and hapln1a⁺ cells in the wound area of adult injured deltaC:EGFP;hapln1a:mCherry animals. White dashed box is enlarged in single confocal slices. Arrows label the coronary tips. White dashed line circles the closing wound. n = 10 animals. Scale bars, 50 µm. b 3-D visualization of coronary sprouts and hapln1a⁺ cells in the regenerating area in experiment (a). Arrow represents the coronary tip lagging behind hapln1a⁺ cells. Scale bars, 50 µm. c Section views of coronary vessels in the wounded area in adult injured deltaC:EGFP;hapln1a:mCherry animals and deltaC:EGFP siblings, with Mtz treatment by 30 dpa. Brackets represent the regenerated area. Scale bars, 50 µm. d Quantification of EGFP⁺ pixels in the regenerating site from (c). n = 18 deltaC:EGFP and n = 22 deltaC:EGFP;hapln1a:mCherry animals were used. The experiments were repeated at least once. Mann–Whitney rank-sum test (Two-sided). Data are presented as mean values ± SD. Source data are provided as a Source Data file.

Acknowledgments

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