Fig. 6.

Cell-pair asymmetry affects the precision and accuracy of Phase 3. (A) Relationship between the final and initial angles. The initial angle is the positional angle of the cell pair immediately after the division. The final angle is where cells come to rest (regardless of whether they have rotated). Note that both angles were normalized to 0-180° (from 0-360°). (B) Relationship between the initial angle and the final turn of cell pairs of wild type, emx2 mutant and notch1a mutants. Note the initial angle was normalized to 0-180° (from 0-360°). (C) Comparison of the final turn of cell pairs from wild type, emx2 mutant and notch1a mutant larvae. Statistics were calculated using an unpaired two-sided Student's t-test. Box plots show median values (middle bars) and first (Q1) to third (Q3) interquartile ranges (boxes); upper whisker is either 1.5× the interquartile range or the maximum value (whichever is the smallest) and lower whisker is either 1.5× the interquartile range or the minimum value (whichever is the biggest). Each point represents the cells during a rotation. (D) Final angle of cell pairs from wild type, emx2 and notch1a mutants. The difference in the distribution of final angles from wild type and the two mutants are statistically significant (P<0.05), Statistics were calculated using a two sample Kolmogorov–Smirnov test (P=0.004 for wild type and emx2 mutant, and P=0.01 for wild type and notch1a mutant). (A-D) n (number of cell pairs)=71 from wild type, 42 from emx2 mutant, 22 from notch1a mutants.