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Figure 6
O. alcalica MyoD1 and D. rerio MyoD1 activate muscle gene expression. (A) The expression of myosin 4 (myh4) is quantified by qRT-PCR analysis of RNA extracted from explants expressing O.alcalica MyoD1 compared to D. rerio MyoD1. (B) The expression of actin 3 (act3) is quantified by qRT-PCR analysis of RNA extracted from explants expressing O.alcalica MyoD1 compared to D. rerio MyoD1. While both MyoD1 proteins can activate high levels of contractile gene expression in naive tissue, there is a significantly higher response to D. rerio MyoD1. Experiment was carried out in triplicate and average Ct was normalised to the housekeeping gene Dicer. Pairwise t-tests comparing the mean relative expression for control and experimental sets for each gene. Error bars represent SEM, * = p < 0.05.