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Figure 5
Fusogenic liposomes mediate efficient expression in zebrafish embryos. GFP-mRNA was injected into the zebrafish yolk at 4.5 hpf by endosome-independent (fusion) and endosome-dependent (lipofection) mechanisms. Embryos were analyzed for GFP expression 24 h (fusion) and 48 h (lipofection) after injection. eGFP-mRNA was used in a concentration of 0.3 ng/µL (corresponds to 1.5 ng per injection). Scale bar = 200 µm.
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