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Figure 10. (A) Activities of lipase (n=6 for each experimental group) and (B) expressions of LIP (n=3 for each experimental group) in zebrafish livers after 28-d exposure to control, MPs, OTC, and MP-OTC, respectively. The activity of lipase was determined with the commercial lipase activity kit (BC2345; Solarbio) using a microplate reader (Multiskan GO; Thermo Scientific) and LIP expression was determined by real-time PCR with a Bio-Rad real-time system (CFX96; Bio-Rad). The corresponding numeric data are provided in Table S13. Data (means±SEs) with different superscripts above were significantly different at p<0.05 (one-way analysis of variance and Tukey’s post hoc tests). Note: LIP, gene encoding lipase; MP-OTC, microplastics and oxytetracycline; MPs, microplastics; OTC, oxytetracycline; PCR, polymerase chain reaction; prot, protein; SE, standard error.