Figure 5

In vivo effect of PDS on pre-miR-150 G4 during zebrafish embryo development. RT-qPCR quantification of miR-150 (a) and myb (b) of 24 hpf staged embryos microinjected with G- or 7DG-miR-150 in the absence or in the presence of 5 µM PDS. Ordinary 1-way ANOVA with Tukey multiple comparisons test, p < 0.001. Trunk length (c) and eye size (d) measurements of microinjected 48 hpf staged larvae microinjected with G- or 7DG-miR-150 in the absence or in the presence of 5 µM PDS. Ordinary 1-way ANOVA with Tukey multiple comparisons test, p < 0.001. Letters above each condition in the graphs represent statistical groups, where means with no letter in common are significantly different. For graphs shown in (a–d), p-values are detailed in Supplementary Table S7 and numerical values are detailed in Supplementary Table S8. (e) Micrographs of representative 48 hpf staged larvae (lateral view, head to the left) microinjected with the RNA transcribed from pSP64T+dsRED plasmid containing no cloned pre-miRNA (control), G-pre-miR-150, G-pre-miR-150 + PDS, 7DG-pre-miR-150, and 7DG-pre-miR-150 + PDS. The measured parameters for graphs in Figure 5c,d are indicated by blue, dashed lines in the control. Data shown represent results of one from three independent experiments.