ZFIN Image: Steeman et al., 2023, Figure 2

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Figure Caption

Figure 2

In vitro evidence of the formation of a G4 structure in the PQS present in pre-miR-150. (a) CD spectroscopy of the folded RNA oligonucleotide containing the pre-miR-150 PQS in different K⁺ and Li⁺ concentrations. (b) TDS spectrum of the RNA oligonucleotide containing the pre-miR-150 PQS (70 °C spectrum–20 °C spectrum) folded in the presence of 1 mM K⁺. (c) ThT fluorescence assay with the RNA oligonucleotide containing the pre-miR-150 PQS folded in the presence of 1 mM K⁺. Two-tailed t-Student, *** indicates p < 0.0001. (d) Imino proton region of the 1D ¹H NMR spectrum of the RNA oligonucleotide containing the pre-miR-150 PQS folded in the presence of 1 mM K⁺.

Acknowledgments

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