ZFIN Image: Schaefer et al., 2023, Figure 2

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Figure Caption

Figure 2

Use of recreational LED flashlights and theater stage lighting filters for smartphone green fluorescence microscopy. (a) Schematic of components used for green fluorescence viewing on the glowscope. (b–d) Representative fluorescence images of transgenic zebrafish embryos (3–4 dpf, lateral views) expressing green fluorescence in cell-type specific patterns. Reporter lines viewed include Tg(nkx2.2a:memGFP) (b), Tg(myl7:EGFP) (c), and Tg(phox2b:EGFP) (d). (e) Image shows the glowscope in use for green fluorescence viewing. (f–g) Plots show the blue LED flashlight emission wavelength (black and blue lines, measured using a Vernier spectrometer) in comparison to the excitation profile of EGFP (gray, obtained from www.fpbase.org). The dashed box region in panel (f) is further magnified in panel g to show the effect of the R4990 filter with greater detail. Images in panels (b–d) were acquired using an Apple iPhone XR.

Acknowledgments

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