ZFIN Image: Balamurugan et al., 2022, Fig. 3

Image

Figure Caption

Fig. 3

RNA-seq analysis of PHLPP1 depleted in vitro foam cells

(A) Representation of experimental conditions-set 1: control cells versus OxLDL-treated control cells; set 2: control cells versus PHLPP1-knockdown cells; set 3: OxLDL-treated control cells versus OxLDL-treated PHLPP1-knockdown cells.

(B) Volcano plot analysis of set 1, 2 and 3 genes.

(C) Venn diagram showing commonly affected genes in the three sets.

(D) Top 25 affected pathways in set 3 were identified using Enrichr tool by submitting the differentially altered genes from set 3 (fold change >1.5, FDR: 0.05). KEGG 2021 human pathways library was used to analyze the possible disease conditions.

(E) Heatmap analysis of selected pathways (inflammation, cholesterol biosynthesis, and lipid metabolism) of set 3. Data are a representative of three independent experiments. See also Figure S3.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ iScience