Image
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Figure Caption
Fig. S1 Generation of the pDestTol2CG2_3×PRE-ZOP:SF-whrna expression construct. A 3× photoreceptor regulatory element (PRE)-containing zebrafish rod opsin promoter element (ZOP), a double Strep and single FLAG-tag (Strep/FLAG) and whrna (ENSDART00000111659.4) were cloned into the pDestTol2CG2 destination vector. This resulted in the pDestTol2CG2_3xPRE-ZOP:SF-whrna expression construct, which further contains the heart-specific cmcl2 promoter driving the expression of EGFP.
Figure Data
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Construct:
Tg(rho:SFtag-whrna,myl7:EGFP)
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Acknowledgments
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Reprinted from Journal of proteomics, 266, Schellens, R.T.W., Slijkerman, R.W.N., Hetterschijt, L., Peters, T., Broekman, S., Clemént, A., Westerfield, M., Phillips, J.B., Boldt, K., Kremer, H., De Vrieze, E., Van Wijk, E., Affinity purification of in vivo assembled whirlin-associated protein complexes from the zebrafish retina, 104666, Copyright (2022) with permission from Elsevier. Full text @ J. Proteomics