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Figure 3
Effect of G-CSFR truncation mutation on definitive hematopoiesis. Wild-type (wt/wt), heterozygous (wt/mdu26) and homozygous (mdu26/mdu26) mutant csf3r embryos were subjected to WISH at 5 dpf with mpo (A–C), mpeg1.1 (E–G), rag1 (I–K) and hbbe1.1 (M–O), with representative images shown. Individual embryos were assessed for the number of mpo+ (D) or mpeg1.1+ (H) cells or the area of staining for rag1 (L) or hbbe1.1 (P), with mean and SEM shown in red and level of statistical significance indicated (***: p < 0.001, ns: not significant; n=20-28).
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