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Figure 5.
Reissner fiber (RF) breakdown in cfap298tm304 mutants but not urotensin-deficient mutants.
(A–F) Grayscale maximal intensity projection of Sspo-GFP localization in the central canal in 28 hpf embryos (A–C) and 12 dpf adolescents (D–F). RF is denoted by arrow heads in D and F. Arrows point to structures along the central canal that become GFP-positive in cfap298tm304 and uts2r3b1436 mutants. Scale bar: 10 µm. (G) Schematic of temperature shift experiment in which cfap298tm304 mutants are initially raised at permissive temperatures before being shifted to restrictive temperatures at 6 dpf, then imaged at 12 dpf. (H–I) Lateral views of cfap298tm304 (H) and uts2r3b1436 (I) mutants at 12 dpf when Sspo-GFP imaging took place. The white box in H shows the location imaged in D–F.