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FIGURE 3

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ZDB-IMAGE-221227-5
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Figures for Sun et al., 2022
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FIGURE 3

Craniofacial phenotype characterization in chd7 sr5 mutants. (A) Viscerocranium morphology. The viscerocranium was dissected out from the Alcian blue and Alizarin red costained WT, chd7 sr5, and MZchd7 sr5 mutants with or without heart edema at 7 dpf. Yellow arrowheads indicate the reduction in the IV ceratobranchial; black asterisks mark the defects in the teeth; red asterisks mark the abnormalities in the basihyal. (B) Quantification of the different types of the morphology of basihyal in WT, chd7 sr5, and MZchd7 sr5. (WT: n = 16; chd7 sr5 : n = 16; MZchd7 sr5 : n = 15). The statistical analysis was performed using the chi-square test. (C) Schematic of the viscerocranium cartilage at 7 dpf. bb, basibranchial; bh, basihyal; cb, ceratobranchial; ch, ceratohyal; hb, hypobranchial; hs, hyosymplectic; ih, interhyal; m, Meckel’s; and pq, palatoquadrate. (D) Quantification of the length of I to V ceratobranchials in WT, chd7 sr5, and MZchd7 sr5 . (WT: n = 24; chd7 sr5 : n = 20; MZchd7 sr5 : n = 23). The statistical significance was performed using the ordinary one-way ANOVA test. (E) Quantification of different types of the morphology of the IV ceratobranchial. (n = 22 for each group). The statistical analysis was performed using the chi-square test. (F) High-magnification image shows that chondrocyte stacking is disrupted in chd7 sr5 mutants, and severely disorganized stacking has been found in MZchd7 sr5 mutants. (G) Schematic of chondrocytes’ stacking measurement. (H) Angles of three neighbor chondrocytes in I and II ceratobranchials. Each number indicates a different fish. (n = 6 for each group). The significance was calculated between different groups. chd7 sr5 mutants have mild disorganization in the chondrocyte arrangement, but MZchd7 sr5 mutants have more severe defects in the chondrocytes. The statistical analysis was performed using the ordinary one-way ANOVA test. (I) Alizarin red-stained teeth are reduced in chd7 sr5 mutants at 7 dpf. Nearly 50% of MZchd7 sr5 fish have defects in teeth number. (WT: n = 23; chd7 sr5 : n = 20; MZchd7 sr5 : n = 38). The statistical significance was performed using the chi-square test. ns: p ≥ 0.05, *: p < 0.05, **: p < 0.01, ***: p < 0.001, and ****: p < 0.0001.

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