Fig. 2

Fig. 2 Fig. 2. Localized patterning of LWS1 cones in control and regenerated retinas of adult zebrafish at 21 days following ouabain lesion. A. Representative regions sampled for nearest neighbor distance (NND) analysis from control (Con; A) and contralateral regenerated (Reg; A′) retinas of lws:PAC(H) zebrafish, in which lws1 is reported by GFP (LWS1 cones; green), counterstained with DAPI (blue). Scale bar (applies to A, A′) = 10 μm. Inset in A (same magnification) shows LWS2 cone reporter dsRedExpress forming aggregates in undamaged retina, making the identification of the location of individual LWS2 cones extremely difficult. An example of three aggregates that may or may not be localized to a single cone is encircled. B. Scatterplots show NNDs of individual LWS1 cones in one of three representative regions of four sampled control retinas (blue symbols) and contralateral regenerated retinas (orange symbols). Each graph describes one of the three sampled regions from contralateral retinas. C. Distribution of average NNDs from regions sampled show decreased NNDs of LWS1 cones in regenerated retinas (**p < 0.01; Student's t-test). D. Conformity ratios of the LWS1 pattern are decreased in regenerated retinas (***p < 0.001; Mann-Whitney test), indicating decreased pattern regularity. E. Density of LWS1 cones in the regions sampled are not different in control vs. regenerated retinas. In the boxplots of C-E, the boxes demarcate the 25th and 75th percentiles, the horizontal lines within the boxes designate the medians, the “X” designates the means, and the whiskers represent the upper and lower limits. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)