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Fig. 5

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ZDB-IMAGE-221208-16
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Figures for Campbell et al., 2021
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Fig. 5

TFAP2 correlates with clustering in human melanoma and regulates genes associated with metastasis and cell-cell adhesion

a. Human melanoma cell lines in the Cancer Cell Line Encyclopedia (CCLE, n=56) plotted as PRO and INV scores (Hoek et al., 2006) and colored according to TFAP2A mRNA expression. Pearson correlation coefficients between TFAP2A and PRO/INV scores are shown on axes. b. TFAP2A mRNA expression in The Cancer Genome Atlas (TCGA) melanoma (SKCM) cohort comparing primary tumors and metastases (p<0.001 by Wilcoxon rank sum test with Bonferroni correction). c. Low-passage human melanoma cell lines ranked by increased cluster forming ability (left to right) with TFAP2A expression quantified by immunofluorescence (plot and top; Spearman correlation shown; scale bar 20 μm) and clustering (bottom, scale bar 500 μm). d. GSAA was run using gene sets and GO gene sets with FDR < 0.05 from INV vs. PRO RNA-seq (n=39 gene sets; cyan points in Figures 1d and and2a).2a). Bars show Normalized Association Score (NAS) for CRISPR (ZMEL1-PRO sg_tfap2a/e vs. sg_scr) and INV vs. PRO for each gene set, with black outline representing FDR<0.05 for CRISPR experiment. e. Plot of INV signature (Hoek et al., 2006) by GSAA for ZMEL1-PRO sg_tfap2a/e vs. sg_scr RNA-seq. f. Heatmap of top genes in Hoek INV and GO Adhesion gene sets that are differentially expressed between ZMEL1-PRO sg_tfap2a/e and sg_scr (log2 fold change cutoff ± 0.5, padj < 0.05). Asterisks (*) indicate genes with associated TFAP2A CUT&RUN peaks. Human ortholog gene names are used for clarity (see Figure S5p for zebrafish gene names). See also Figure S5 and Tables S4,S5,S6.

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Reprinted from Developmental Cell, 56(20), Campbell, N.R., Rao, A., Hunter, M.V., Sznurkowska, M.K., Briker, L., Zhang, M., Baron, M., Heilmann, S., Deforet, M., Kenny, C., Ferretti, L.P., Huang, T.H., Perlee, S., Garg, M., Nsengimana, J., Saini, M., Montal, E., Tagore, M., Newton-Bishop, J., Middleton, M.R., Corrie, P., Adams, D.J., Rabbie, R., Aceto, N., Levesque, M.P., Cornell, R.A., Yanai, I., Xavier, J.B., White, R.M., Cooperation between melanoma cell states promotes metastasis through heterotypic cluster formation, 2808-2825.e10, Copyright (2021) with permission from Elsevier. Full text @ Dev. Cell