Fig. 5

Fig. 5. Imaging the neuronal activity of a larval zebrafish brain expressing pan-neuronal GCaMP7a using 3DM. (a) Three z-slices (at z=70 $\mathrm {\mu }$m, 100 $\mathrm {\mu }$m, 140 $\mathrm {\mu }$m, counted upwards from top to bottom, z = 0 $\mathrm {\mu }$m indicates the top surface of the brain) from the deconvolved low rank image (left). Enlarged views of the boxed area with the neuronal activity superimposed on the low rank image at multiple time points (right). Scale bar, 50 $\mathrm {\mu }$m. (b) Extracted neuronal activities shown as a heat map. (c) Randomly selected neurons and the corresponding extracted neuronal activity. The color of each selected neuron is matched to that of the corresponding activity. Scale bar, 50 $\mathrm {\mu }$m.