FIGURE 6

FIGURE 6

Behavioral analysis of 6 dpf zebrafish larvae treated with orexin receptor antagonists in the dark-light assay. Locomotor profiles of animals are expressed as mean actinteg units per 1 min treated with suvorexant (A), TCS-1102 (C) and SB-674042 (E) are plotted as function of time. For the sake of clarity, standard deviations are not shown. Different phases are shown, i.e., 30 min light phase (Light), the first 15 min of the dark phase (Dark 1) and the last 15 min of the dark phase (Dark 2). Total locomotor activity was averaged (±SD) per respective light or dark phase (B–F). For each compound, data were pooled from three or nine independent experiments with 9–20 replicate wells per test condition (n = 29–40, except for VHC (C,D)n = 110) and 30 µM TCS-1102 (n = 50)). Statistical analysis: two-way ANOVA with Dunnett’s multiple comparison test. Significance levels: *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.