ZFIN Image: Field et al., 2022, FIGURE 2

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FIGURE 2

Tmem184a KD Results in Decreased Number of Intact Intersegmental Vessels. (A,A′) SC MO injected embryo (n = 35). (B,B′) Tmem184a SB MO injected embryo (n = 25). (C,C′) Tmem184a ATG MO injected embryo (n = 19). Asterisks indicate ISVs that failed to complete injected embryos. (D,D′) Injection with full-length Tmem184a mRNA alone (n = 21). (E,E′) Co-injection with full-length Tmem184a mRNA rescues vascular defects in Tmem184a SB MO injected embryos (n = 15). (A′–E′) illustrate zoomed trunk regions of the fish. (F). KD by SB and ATG MOs compared to SC MO embryos and rescue embryos with SB MO and Tmem184a mRNA, illustrating the mean number of intact ISVs ± SEM. p < 0.001. (G) Embryo survival for all treatments shown as compared to SC embryo survival at 24 h ± SEM p < 0.001.

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Acknowledgments

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