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Fig. 1
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Figure Caption
Fig. 1. WT S1 is highly proinflammatory in zebrafish.
Recombinant S1WT (+), BSA, or vehicle (−) were injected in the hindbrain ventricle (HBV) of 2–day postfertilization (dpf) Tg(mpx:eGFP) (A), Tg(mfap4:mCherry) (B), Tg(nfkb:eGFP) (C), and WT (D to F) larvae. Neutrophil (A) and macrophage (B) recruitment and number and Nfkb activation (C) were analyzed at 6, 12, and 24 hpi by fluorescence microscopy, the transcript levels of the indicated genes (D) were analyzed at 12 hpi by RT-qPCR in larval head and tail (except for ifng that was also analyzed at 6 hpi in the head) (E), and caspase-1 activity was determined at 24 hpi using a fluorogenic substrate (F). As a control, S1WT was also used preheated at 80°C for 30 min (S1-80). Each dot represents one individual, and the means ± SEM for each group is also shown. P values were calculated using one-way analysis of variance (ANOVA) and Tukey multiple range test. RT-qPCR data are depicted as a heatmap in (D) with higher expression shown in darker color. ns, not significant; *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001. auf, arbitrary units of fluorescence.
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