ZFIN Image: Moret et al., 2022, Fig. 4

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Fig. 4

Cytotoxicity of DTX-loaded NPs in KB spheroids. A Percentage of residual ATP in the spheroid measured using the CellTiter-Glo® 3D Cell Viability Assay after 72 h of treatment with DTX-loaded NPs or with the free drug ([DTX] = 0.001–1 μg/mL). Data are mean values ± SD of at least three independent experiments carried out in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001 vs. free DTX (Student’s t-test). B Bright-field images of spheroids acquired by confocal microscopy after 72 h of treatment with DTX-loaded NPs at different doses. Scale bars: 100 μm

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