ZFIN Image: Zhuang et al., 2022, Fig. 6

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Fig. 6

Histone deacetylases and NF-κB signaling cooperate to promote repair through migration. (A) Whole-mount in situ hybridization reveals that nfkbiaa is upregulated in the cells flanking the injury site and the expression is persistent in the first 4 h post injury (hpi). (B) An example of nfkbiaa SBM-injected embryo 24 h post ablation. The white arrow points to the injury site. (C) Depletion of nfkbiaa with splice blocking MO (SBM) significantly reduced the number of successful repairs. (D) nfkbiaa TBM-injected embryos repair at significantly lower rate than controls. (E) Combined depletion of hdac6 TBM, hdac8 TBM, and nfkbiaa TBM with low MO concentrations synergistically reduces the number of embryos that successfully repair the pronephros. The number of examined embryos is shown in brackets. The points represent individual experiments. Mean and standard deviation for each group are displayed. Significance was calculated with Fisher’s exact test (two-tailed).

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Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.