|
Fig. 12
Experimental design. Using the alternating, increasing immersion protocol, hyperglycemia was induced in adult zebrafish for either 8 weeks (8-week FULL) or 12 weeks (12-week FULL). To assess the effect of recovery, additional experiments were performed in which the fish were hyperglycemic for 4 weeks, followed by 4 weeks removed from treatment (8-week WASHOUT) or hyperglycemic for 8 weeks, followed by 4 weeks removed from treatment (12-week WASHOUT). Each arrow in the above figure represents 4 weeks; darker arrows reflect treatment exposure, and thin arrows represent recovery. At each 4-week timepoint, cognitive behavior was assessed using the three-chamber choice task (3CC). At 8 weeks and 12 weeks, optomotor responses (OMRs) were also recorded.