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Fig. 3 Figure 3. Distribution of reticulospinal neurons and excitatory and inhibitory neuronal cell types in the hindbrain of DC and ZF (A) Distribution of reticulospinal (RS) neurons in the brainstem of DC. Maximum intensity is color coded for depth. Scale bar, 100 μm. (B) Comparison of RS neurons in DC and ZF. A cell-to-cell comparison of RS neurons in a maximum intensity projection of RS neurons in DC and ZF. The RS neurons in DC are annotated based on the description of RS neurons in ZF (Kimmel et al., 1982; Orger et al., 2008). A high degree of conservation is observed. Scale bar, 100 μm. (C) A closer look at the rectangular region of interest (ROI) from (B) in another DC fish. Maximum intensity projection of the nucleus of the medial longitudinal fasciculus (nMLF) in another DC fish is shown. Contralateral dendritic projections are observed in DC as noted in ZF (Kimmel et al., 1982). Scale bar, 30 μm. (D–F) Distribution of (D) glutamatergic, (E) glycinergic, and (F) GABAergic neurons in the hindbrain of DC. Performed using in situ hybridization (ISH) and immunohistochemistry (IHC). Rostrocaudally running striped pattern of these neuronal types is observed in DC as noted in ZF before (Higashijima et al., 2004). Anti-vglut2a + anti-vglut2b ISH and anti-glyt2 ISH in (D and E), respectively. Anti-GAD65/67 IHC in (F). All images are a maximum intensity projection. Scale bar, 50 μm. See also Figure S3.