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Fig. 1
Fig. 1. Sex ratio response to temperature in the AB, EKW and NA strains. Percent male for the 28 vs. 36 °C comparison from three independent families each of A) AB strain (AB#1, solid square: n♂/♀ = 31/26 vs. 33/5; AB#2, solid circle: n♂/♀ = 9/23 vs. 31/10 and AB#3, solid triangle: n♂/♀ = 17/15 vs. 34/3 fish). B) EKW strain (EKW#1, solid square: n♂/♀ = 16/38 vs. 40/13; EKW#2, solid circle: n♂/♀ = 20/26 vs. 53/1 and EKW#3, solid triangle: n♂/♀ = 39/16 vs. 47/4 fish. C) Percent male from six independent NA families: three for the 28–34 °C comparison (NAD#4 open square: n♂/♀ = 45/13 vs. 46/14; NAD#5 solid triangle: n♂/♀ = 40/16 vs. 59/1 and NAD#6 open triangle: n♂/♀ = 51/6 vs. 54/4 fish) and three for the 28–36 °C comparison (NAD#1, open circle: n♂/♀ = 38/16 vs. 48/0; NAD#2, solid square: n♂/♀ = 48/9 vs. 57/0 and NAD#3, solid circle: n♂/♀ = 37/17 vs. 48/0 fish). A Fisher's exact test was applied individually for each family comparing phenotypic proportions of males and females between treatments. In all strains, each family was split into three tanks per temperature (technical replicate). Solid and open points are used only for improved clarity due to overlapping symbols. Abbreviations: * = P < 0.05; ** = P < 0.01; *** = P < 0.001; N.S. = not significant.