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Fig. 2
Fig. 2. Western blot analysis of FLAG-tagged proteins upon anti-FLAG affinity purification of wild-type and SF-whrna zebrafish retinal extracts. SF-Whrna (83 kDa, indicated with an arrow) was abundantly detected in the SF-whrna retinal extract (input) and in the SF-whrna eluate after affinity purification, but little was detected in the SF-whrna flow-through upon washing. In the wild-type samples, SF-Whrna was not detected.
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Reprinted from Journal of proteomics, 266, Schellens, R.T.W., Slijkerman, R.W.N., Hetterschijt, L., Peters, T., Broekman, S., Clemént, A., Westerfield, M., Phillips, J.B., Boldt, K., Kremer, H., De Vrieze, E., Van Wijk, E., Affinity purification of in vivo assembled whirlin-associated protein complexes from the zebrafish retina, 104666, Copyright (2022) with permission from Elsevier. Full text @ J. Proteomics