Figure 2

Figure 2

The z-Idua enzymatic activity and occurrence of defective phenotypes in zebrafish embryos was impacted by injection of mutated z-idua mRNA into embryos. (A) The z-Idua enzymatic activity of untreated zebrafish embryos at 24 hpf, which served as the control group, was quantified and normalized as 1 for comparison with the relative z-Idua activity obtained from the other experimental groups. The z-Idua enzymatic activity of embryos injected with 100-pg zebrafish idua mRNA (z-idua; served as positive control) and various point-mutated z-idua mRNAs, as indicated, were quantified. Each point-mutation that occurred in z-idua corresponded with that of human MPS I patients. (B) Embryonic phenotypes were examined at the 120 hpf zebrafish embryos injected at one-cell stage with 100-pg zebrafish idua mRNAs (z-idua), including mutated variants, as indicated. Each mutated zebrafish idua.