ZFIN Image: Liao et al., 2021, Fig. 7

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Fig. 7

Schematic model of the regulation of granulopoiesis and neutrophil functions by VD₃ in zebrafish. ① In adult zebrafish, the gene expression of csf3b and the neutrophil markers were enhanced in the intestine of the fish fed with VD₃-containing diet, compared to those fed with non-VD₃ diet. ② The gene expression of csf3b and neutrophil abundance in the intestine of adult zebrafish with a cyp2r1 mutant were reduced. Interestingly, microbiota is involved in VD-mediated granulopoiesis in adult zebrafish. ③ Addition of exogenous VD₃ promoted granulopoiesis in zebrafish larvae. However, VD-regulated neutrophil generation independent of the microbiota during early development. ④ VD₃ treatment significantly decreased bacterial counts and mortality in zebrafish larvae infected with E. tarda in a neutrophil-dependent manner. ⑤ VD₃ augmented the gene expression of IL-8 and neutrophil recruitment to the site of caudal fin amputation. csf3b, colony-stimulating factor 3b; VD_3, vitamin D₃; E. tarda, Edwardsiella tarda.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Innate Immun.