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Fig. 4 Extracted ion chromatograms of metabolite standards: hydrocortisone (1), tyramine (2), serotonin (3), 2′-deoxyguanosine (4), tryptophan (5), 3,4-dihydroxyphenylacetic acid (6), biotin (7), pantothenic acid (8), mannose (9), lauric acid (10), N-acetyl-L-aspartic acid (11), caprylic acid (12), maleic acid (13), and taurocholic acid (14); analyzed by the HILIC column with different solvent additives in negative ionization mode. Under different condition groups (a) and (b) include metabolites with relatively low and high ESI intensity, respectively.
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