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Fig. 1
Expression of the zygotic gene, isg15, in zebrafish embryos after invasion by the plasmid, pEGFP-N1. A Heatmap to show normalized expression count differences of immune related genes from transcriptome sequencing at 6 hpf after injection of the foreign plasmid, pEGFP-N1, into 1-cell stage embryos. B Expression levels of six genes related to the apoptosis pathway, endogenous immune response and tumor necrosis factor-mediated pathway are up-regulated. C Up-regulation of isg15 expression in 6 hpf zebrafish embryos by qRT-PCR. D Expression of isg15 in 8-cell stage and High stage embryos after plasmid injection compared with controls. 8 pg, 32 pg, 128 pg: the injected amount of plasmid pEGFP-N1. NC: un-injected zebrafish embryos